Up-Regulated Gene 7 (Urg7) encodes a protein 99 amino acids long that presents a high homology with the protein product of the pseudogene 2 (ABCC6Ψ2) of the ABCC6 gene [1]. Is known that URG7 contributes to the survival of HBV-infected hepatocytes during chronic infection. In particular, HBxAg activation of the proinflammatory NF-KB results in the up-regulation of URG7 that inhibits TNFα-mediated killing by inhibiting caspases 3 and 8 activity in the apoptotic pathway and by activating phosphoinositol 3-kinase and β-catenin [2]. In order to obtain information about the structure and function URG7 has been expressed in E.coli and purified by RP-HPLC. CD spectroscopy studies performed in the far UV (190-250 nm) have shown a heterogeneous population of α-helice and β -secondary structures. Moreover, URG7 has been expressed in E.coli with 15N isotope-labelling for further structural characterization by NMR spectroscopy. [1] L. Pulkkinen, A. Nakano, F. Ringfeil. Identification of ABCC6 pseudogenes on human chromosome 16p: implications for mutation detection in pseudoxanthoma elasticum. Hum Gent,(2001) 109, 356-365. [2] J. Pan,Z. Lian, S.Wallet and M.A. Feitelson. The hepatitis B x antigen effector, URG7, blocks tumour necrosis factor a-mediated apoptosis by activation of phosphoinositol 3-kinase and β-catenin. Journal of General Virology (2007), 88, 3275–3285.
Structural studies on up-regulated gene 7 (urg7)
SALVIA, ANTONELLA MARIA;MIGLIONICO, ROCCHINA;OSTUNI, Angela;CASTIGLIONE MORELLI, Maria Antonietta;ARMENTANO, MARIA FRANCESCA;BISACCIA, Faustino
2012-01-01
Abstract
Up-Regulated Gene 7 (Urg7) encodes a protein 99 amino acids long that presents a high homology with the protein product of the pseudogene 2 (ABCC6Ψ2) of the ABCC6 gene [1]. Is known that URG7 contributes to the survival of HBV-infected hepatocytes during chronic infection. In particular, HBxAg activation of the proinflammatory NF-KB results in the up-regulation of URG7 that inhibits TNFα-mediated killing by inhibiting caspases 3 and 8 activity in the apoptotic pathway and by activating phosphoinositol 3-kinase and β-catenin [2]. In order to obtain information about the structure and function URG7 has been expressed in E.coli and purified by RP-HPLC. CD spectroscopy studies performed in the far UV (190-250 nm) have shown a heterogeneous population of α-helice and β -secondary structures. Moreover, URG7 has been expressed in E.coli with 15N isotope-labelling for further structural characterization by NMR spectroscopy. [1] L. Pulkkinen, A. Nakano, F. Ringfeil. Identification of ABCC6 pseudogenes on human chromosome 16p: implications for mutation detection in pseudoxanthoma elasticum. Hum Gent,(2001) 109, 356-365. [2] J. Pan,Z. Lian, S.Wallet and M.A. Feitelson. The hepatitis B x antigen effector, URG7, blocks tumour necrosis factor a-mediated apoptosis by activation of phosphoinositol 3-kinase and β-catenin. Journal of General Virology (2007), 88, 3275–3285.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
