Supercritical fluid extraction (SFE) has been combined with supercritical fluid chromatography SFC) in a preparative mode to develop a system for fractionating and enriching high value constituents contained in seed oil matrioces. The system consists of an extraction step sequenced on-line with a sorbent filled column, wich permits a SFE-enriched tocoppherol fraction to be diverted onto the chromatographic column for further enrichment of the tocopherols. For the SFE stage the tocopherol enrichment was oprimized at 25 MPa and 80 °C for spybean flakes and rice bran. However, total tocopherol recovery and enrichment was also found to be a critical function of the mass ratio CO2/seed charge. Approximately 60% of the available tocopherols in spyflakes can be recovered in the SFE step, yielding enrichment factors of 1.83-4.33 for the four tocfopherol pecies found in soybean oil. Additional enrichment species can be realized in the SFC stage, ranging from 30.8 for delta tocopherol to 2.41 for beta tocopherol.

Production of Tocopherol Concentrate by Supercritical Fluid Extraction and Chromatography.

FAVATI, Fabio;
1996

Abstract

Supercritical fluid extraction (SFE) has been combined with supercritical fluid chromatography SFC) in a preparative mode to develop a system for fractionating and enriching high value constituents contained in seed oil matrioces. The system consists of an extraction step sequenced on-line with a sorbent filled column, wich permits a SFE-enriched tocoppherol fraction to be diverted onto the chromatographic column for further enrichment of the tocopherols. For the SFE stage the tocopherol enrichment was oprimized at 25 MPa and 80 °C for spybean flakes and rice bran. However, total tocopherol recovery and enrichment was also found to be a critical function of the mass ratio CO2/seed charge. Approximately 60% of the available tocopherols in spyflakes can be recovered in the SFE step, yielding enrichment factors of 1.83-4.33 for the four tocfopherol pecies found in soybean oil. Additional enrichment species can be realized in the SFC stage, ranging from 30.8 for delta tocopherol to 2.41 for beta tocopherol.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11563/3973
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