Hypoestes forsskaolii is a perennial herb belonging to Acanthaceae family, which shows several beneficial properties as antioxidant, antibacterial, anticancer, antifungal and antiparasitic activity (Mothana et al. 2009; Ojo-Amaize et al. 2007; Rasoamiaranjanahary et al. 2003). Plants produce wide array of bioactive molecules, most of which probably evolved as chemical defense against predation or infection (Samie et al. 2010). A medicinal plant is any plant which, in one or more of its organ, contains substance that can be used for therapeutic purpose or which is a precursor for synthesis of useful drugs (Edwards 2004). In order to develop innovative antibacterial compounds with potencial applications in preventing microbial proliferation, in this study we evaluated the antibacterial activity of H. forsskaolii against methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33591. The crude extracts of H. forsskaolii were obtained through a polarity solvent maceration process (n-hexane, chloroform, chloroform : methanol (9:1 v/v) and methanol of 326 g of roots. The extract concentrations used for this study ranged from 50 µg/mL to 500 µg/mL. The antimicrobial activity was measured by suspending MRSA (one single porous bead, a carrier to support microorganisms in Microbank vial containing cryopreservative) in Brain Heart Infusion Broth (Oxoid) for 24 h at 37°C; thereafter, the Optical Density at 600 nm (OD 600) of the bacterial suspension was adjusted to 0.1 with the addition of sterile broth. Tests were carried out in microtiter plate with the bacterial suspension by adding the extracts at different concentrations (50, 70, 80, 100, 200, 500 µg/mL). The Minimal Inhibitory Concentration (MIC) was defined as the lowest concentration of the extracts able to prevent the microbial growth. The analyses were performed in triplicate on three independent experiments. We demonstrated that only the chloroformic extract was effective in growth inhibition of the tested MRSA strain using a concentration up to 100 µg/mL. The antimicrobial activity reported could be due to the terpenoids which are present in the chloroformic extract. However, further studies are needed to better define the effectiveness of in vitro activity of H. forsskaolii chloroformic extract.

In vitro antibacterial activity of Hypoestes forsskaolii Vahl. Roem & Schult. (Acanthaceae) agaist methicillin-resistant Staphylococcus aureus: Preliminary Results.

Vassallo A.;
2016

Abstract

Hypoestes forsskaolii is a perennial herb belonging to Acanthaceae family, which shows several beneficial properties as antioxidant, antibacterial, anticancer, antifungal and antiparasitic activity (Mothana et al. 2009; Ojo-Amaize et al. 2007; Rasoamiaranjanahary et al. 2003). Plants produce wide array of bioactive molecules, most of which probably evolved as chemical defense against predation or infection (Samie et al. 2010). A medicinal plant is any plant which, in one or more of its organ, contains substance that can be used for therapeutic purpose or which is a precursor for synthesis of useful drugs (Edwards 2004). In order to develop innovative antibacterial compounds with potencial applications in preventing microbial proliferation, in this study we evaluated the antibacterial activity of H. forsskaolii against methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33591. The crude extracts of H. forsskaolii were obtained through a polarity solvent maceration process (n-hexane, chloroform, chloroform : methanol (9:1 v/v) and methanol of 326 g of roots. The extract concentrations used for this study ranged from 50 µg/mL to 500 µg/mL. The antimicrobial activity was measured by suspending MRSA (one single porous bead, a carrier to support microorganisms in Microbank vial containing cryopreservative) in Brain Heart Infusion Broth (Oxoid) for 24 h at 37°C; thereafter, the Optical Density at 600 nm (OD 600) of the bacterial suspension was adjusted to 0.1 with the addition of sterile broth. Tests were carried out in microtiter plate with the bacterial suspension by adding the extracts at different concentrations (50, 70, 80, 100, 200, 500 µg/mL). The Minimal Inhibitory Concentration (MIC) was defined as the lowest concentration of the extracts able to prevent the microbial growth. The analyses were performed in triplicate on three independent experiments. We demonstrated that only the chloroformic extract was effective in growth inhibition of the tested MRSA strain using a concentration up to 100 µg/mL. The antimicrobial activity reported could be due to the terpenoids which are present in the chloroformic extract. However, further studies are needed to better define the effectiveness of in vitro activity of H. forsskaolii chloroformic extract.
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Descrizione: PhOL 2016 - Supplementary Issue - vol. 1, p. 242.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11563/138828
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