Profiling of N-Acyl-Homoserine Lactones by Liquid Chromatography Coupled with Electrospray Ionization and a Hybrid Quadrupole Linear-Ion-Trap and Fourier-Transform Ion-Cyclotron-Resonance Mass Spectrometry (LC-ESI-LTQ-FTICR-MS)

A method for the comprehensive profiling of the N-acyl-homoserine lactone (AHL) family of bacterial quorum-sensing molecules is presented using liquid chromatography (LC) coupled to a hybrid quadrupole linear ion trap (LTQ) and Fourier-transform ion-cyclotron-resonance mass spectrometer (FTICR). We demonstrate an increase in signal intensity in MS with electrospray ionization (ESI) of the protonated molecules, [M + H]+, by using acetonitrile (ACN) instead of methanol (MeOH) as the organic solvent under the conditions in which the samples were supplied to the probe by direct infusion at constant flow rates. The presence of ACN prevents the formation of methanol adducts such as [M + MeOH + H]+ and [M + MeOH + Na]+, while also lowering the signal intensity of sodiated [M + Na]+ ions. Sensitivity of these signaling molecules in terms of signal-to-noise ratio (S/N) using low-resolution LTQ-MS and high-resolution FTICR-MS were compared under reversed-phase (RP) LC separations with ESI interface. Special emphasis was paid to the choice of the separation column, its elution conditions and detection of the major AHL compounds produced by the Serratia liquefaciens strain ATCC 27592. The most promising results were obtained using a RP C16-amide column eluted with a linear mobile phase gradient ACN/H2O containing 0.1% formic acid. The whole set of AHL homologs in bacterial extracts was detected in the extracted-ion chromatographic (XIC) mode, and the calculations of molecular formulae were performed by including the isotopic pattern. This mode of displaying data, with a very narrow mass-to-charge ratio window (i.e. ±0.0010 as m/z unit) around each selected ion, has allowed the identification of all the eight known homoserine lactones, viz. C4-HSL, 3-oxo-C6-HSL, C6-HSL, 3-oxo-C8-HSL, C8-HSL, C10-HSL, C12- HSL andC14-HSL. In addition, at least four uncommon signalingmediators previously unreported, namely, 3-oxo-C10 : 1-HSL, 3-oxo-C11 : 2-HSL, 3-oxo-C13 : 2-HSL and 3-OH-C16-HSL, were identified and characterized; their roles in cell-to-cell communication has to be elucidated.