A simple, rapid and sensitive HPLC method for trace determination of resorcinol in body fluids is described. It uses a reversed phase C18 column and isocratic elution with amperometric detection on glassy carbon at +1.0 V vs. Ag/AgCl reference electrode. 3,5-dihydroxytoluol was used as internal standard. Sample treatment consists of a simple microcolumn extraction step requiring sample volumes as low as 200 microliters. The proposed method gives detection limits (4 ng/mL and 5 ng/mL in serum and urine respectively) about two orders of magnitude lower than other HPLC methods already reported and should prove useful for studying dermal adsorption and metabolic disposition of topically applied resorcinol.

Determination of free resorcinol in human serum and urine by high performance liquid chromatography with amperometric detection

GUERRIERI, Antonio;
1986

Abstract

A simple, rapid and sensitive HPLC method for trace determination of resorcinol in body fluids is described. It uses a reversed phase C18 column and isocratic elution with amperometric detection on glassy carbon at +1.0 V vs. Ag/AgCl reference electrode. 3,5-dihydroxytoluol was used as internal standard. Sample treatment consists of a simple microcolumn extraction step requiring sample volumes as low as 200 microliters. The proposed method gives detection limits (4 ng/mL and 5 ng/mL in serum and urine respectively) about two orders of magnitude lower than other HPLC methods already reported and should prove useful for studying dermal adsorption and metabolic disposition of topically applied resorcinol.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11563/2882
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