Amethopterin has been found to be anodically electroactive on mercury due to a depolarization effect arising from the formation of a sparingly soluble mercury complex. High performance liquid chromatography with anodic polarographic (sampled dc) detection at +0.19 V vs Ag/AgCl has been employed for the determination of the drug in body fluids. Detector response was found to be linear in the range 5 - 1000 ng on column. A detection limit of 1.5 micromolar amethopterin in serum was achieved. The within-day and day-to-day coefficient of variation at the 10 microg/mL level were 1.5 and 3.7 % respectively.

Anodic behaviour of the antineoplastic agent amethopterin at a mercury electrode and its determination in body fluids by liquid chromatographywith indirect anodic polarographic detection

GUERRIERI, Antonio;
1987

Abstract

Amethopterin has been found to be anodically electroactive on mercury due to a depolarization effect arising from the formation of a sparingly soluble mercury complex. High performance liquid chromatography with anodic polarographic (sampled dc) detection at +0.19 V vs Ag/AgCl has been employed for the determination of the drug in body fluids. Detector response was found to be linear in the range 5 - 1000 ng on column. A detection limit of 1.5 micromolar amethopterin in serum was achieved. The within-day and day-to-day coefficient of variation at the 10 microg/mL level were 1.5 and 3.7 % respectively.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11563/2881
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