The anodic behaviour of pseudouridine, a modified nucleoside proposed as tumoral marker, has been studied at a mercury electrode. A method for its determination in urine samples by liquid chromatography with indirect anodic polarographic detection has been developed. The method is simple, high selective and permits the direct injection of urine after dilution (1:20) with mobile phase. Creatinine, which also gave an anodic response on mercury, could be simultaneously determined and used as an "internal standard" for pseudouridine. This allowed the pseudouridine/creatinine ratio determination in a single run on the same specimen and the use of urine samples collected randomly instead of the 24 h collection. The within-day RSD% for pseudouridine/creatinine peak areas ratio was 3.4%.
Simultaneous determination of pseudouridine and creatinine in urine by HPLC with polarographic detection
GUERRIERI, Antonio;
1989-01-01
Abstract
The anodic behaviour of pseudouridine, a modified nucleoside proposed as tumoral marker, has been studied at a mercury electrode. A method for its determination in urine samples by liquid chromatography with indirect anodic polarographic detection has been developed. The method is simple, high selective and permits the direct injection of urine after dilution (1:20) with mobile phase. Creatinine, which also gave an anodic response on mercury, could be simultaneously determined and used as an "internal standard" for pseudouridine. This allowed the pseudouridine/creatinine ratio determination in a single run on the same specimen and the use of urine samples collected randomly instead of the 24 h collection. The within-day RSD% for pseudouridine/creatinine peak areas ratio was 3.4%.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
