The immobilization of glucose oxidase (GOD) in electrochemically synthesized poly-o-phenylenediamine (PPD) film permits the realization of an amperometric biosensor for the determination of glucose in serum by flow injection analysis (FIA). The biosensor was prepared in-situ (i.e. in the FIA system) by injecting a solution containing the monomer and the enzyme. The influence of some experimental parameters (e.g. deposition potential and charge, monomer and enzyme concentration, pH and flow rate) on the sensor's performance has been studied in order to optimize glucose response minimizing, at the same time, interference effects caused by urate, ascorbate, cysteine and acetaminophen. Linearity was observed up to about 15 mM and the lifetime of the sensor ranged within 1-2 weeks. No electrode fouling was observed and undiluted serum could be directly injected. Results for glucose determination in serum compared well (paired t-test at 95 % confidence level) with those obtained by a standard enzymatic-colorimetric method.
An in-situ electrosynthesized poly-o-phenilenediamine/ glucose oxidase amperometric biosensor for flow-injection determination of glucose in serum
GUERRIERI, Antonio;
1992-01-01
Abstract
The immobilization of glucose oxidase (GOD) in electrochemically synthesized poly-o-phenylenediamine (PPD) film permits the realization of an amperometric biosensor for the determination of glucose in serum by flow injection analysis (FIA). The biosensor was prepared in-situ (i.e. in the FIA system) by injecting a solution containing the monomer and the enzyme. The influence of some experimental parameters (e.g. deposition potential and charge, monomer and enzyme concentration, pH and flow rate) on the sensor's performance has been studied in order to optimize glucose response minimizing, at the same time, interference effects caused by urate, ascorbate, cysteine and acetaminophen. Linearity was observed up to about 15 mM and the lifetime of the sensor ranged within 1-2 weeks. No electrode fouling was observed and undiluted serum could be directly injected. Results for glucose determination in serum compared well (paired t-test at 95 % confidence level) with those obtained by a standard enzymatic-colorimetric method.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
