A simple, sensitive and rapid method is described for determining histamine in alkaline media by anion-exchange chromatography with amperometric detection at a copper-based modified glassy carbon electrode. The influence of copper loading, applied potential and flow rate on the amperometric signal was evaluated. Under optimal chromatographic conditions, the detection limit for histamine (S/N = 3) was 0.1 muM (corresponding to 5 pmol injected by a 50-mul sample loop). The linear range spanned from 5 to 500 muM (correlation coefficient = 0.998). The proposed method allows a rapid and convenient quantitation of histamine in real matrices, such as canned tuna and red wine, without time-consuming clean-up or derivatisation procedures.

DETERMINATION OF HISTAMINE BY HIGH-pH ANION-EXCHANGE CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

CASELLA, Innocenzo Giuseppe;
2001-01-01

Abstract

A simple, sensitive and rapid method is described for determining histamine in alkaline media by anion-exchange chromatography with amperometric detection at a copper-based modified glassy carbon electrode. The influence of copper loading, applied potential and flow rate on the amperometric signal was evaluated. Under optimal chromatographic conditions, the detection limit for histamine (S/N = 3) was 0.1 muM (corresponding to 5 pmol injected by a 50-mul sample loop). The linear range spanned from 5 to 500 muM (correlation coefficient = 0.998). The proposed method allows a rapid and convenient quantitation of histamine in real matrices, such as canned tuna and red wine, without time-consuming clean-up or derivatisation procedures.
2001
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11563/2474
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 21
social impact