A disposable amperometric biosensor for the determination of anticholinesterase activity in soil extracts is described. The sensitive membrane was obtained by co-crosslinking acetylcholinesterase and choline oxidase with bovine serum albumin using glutaraldehyde. The anticholinesterase activity of the soil extract was measured using chronoamperometry at 650 mV vs. Ag/AgCl to monitor the biocatalytically produced H2O2 before and after the inhibition step. An inhibition percentage of 38 ± 4% was recorded for soil extracts spiked with 10 ppb of ethyl parathion. The device has the potential to be used as a gross sensor for the assessment of anticholinesterase activity in soil extracts.

A disposable amperometric biosensor for rapid screening of anticholinesterase activity in soil extracts

GUERRIERI, Antonio;
2002-01-01

Abstract

A disposable amperometric biosensor for the determination of anticholinesterase activity in soil extracts is described. The sensitive membrane was obtained by co-crosslinking acetylcholinesterase and choline oxidase with bovine serum albumin using glutaraldehyde. The anticholinesterase activity of the soil extract was measured using chronoamperometry at 650 mV vs. Ag/AgCl to monitor the biocatalytically produced H2O2 before and after the inhibition step. An inhibition percentage of 38 ± 4% was recorded for soil extracts spiked with 10 ppb of ethyl parathion. The device has the potential to be used as a gross sensor for the assessment of anticholinesterase activity in soil extracts.
2002
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11563/2332
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