Relationships between an Italian strawberry ecotype and its ancestor using RAPD markers

Random-amplifiedpolymorphicDNA(RAPD)markerswereusedtoevaluategeneticvariabilityamong populationsofanItalianstrawberryecotype,andtodeterminategeneticrelationshipsbetweengenotypes andtheirputativeancestor.Atotalof65selectionsandonecultivar‘MadameMoutot’(MM),were analysedtoevaluategeneticvariabilitypresentinEtnamountainareaandtoconfirmasMMwasoneof thecultivarsthatoriginatedtheecotype.Atotalof222RAPDmarkerswasobtainedusing16decamer primersand6longerprimers,90.8%ofthemarkersobtainedbyselectedprimersresultedpolymorphicat leastwithinanalysedgenotypes.RAPDswereusedtocalculategeneticsimilaritycoefficientsandtogen- eratedendrogramsrepresentinggeneticrelationshipsamonggenotypesanalysed.Clusteranalysisdisplays asRAPDpolymorphismswereabletocharacterizethegenotypevariabilityamongcloselyrelatedgroups. ThedatashowasMMcouldbeconsideredtheancestralgenotypesintroducedinthatarea.Theresults obtainedconfirmthatRAPDmarkerscouldbeusedasreliablemarkerstoperformphylogeneticstudiesin Fragaria·ananassaDuch.exRozier.