Hura crepitans L. belongs to the family of Euphorbiaceae and it showed many ethnomedicinal applications; the bark extract was used to treat leprosy, eczema and in warfare. Recently a wide range of therapeutic applications of the leaves, stem bark of this plant were reported including astringent, emollient and strong purgative actions, dermatological uses, fungicidal, antimicrobial [1, 2]. The purpose of this study is to increase scientific knowledge on the chemical composition and antioxidant activity of leaves of H. crepitans L. Dried leaves were extracted by different methods: infusion, decoction, maceration by using water as solvent, and sequential extraction by using n-hexane, chloroform, choloform-MeOH 9.1, MeOH was carried out. MeOH extract was partitioned by water and butanol. Antioxidant activity of the extracts was measured by different in-vitro cellfree assays along with the total polyphenolic content and Relative Antioxidant Capacity Index (RACI) was calculated [3]. Aqueous extracts reported the highest RACI values and then, they were tested for the evaluation of cell viability and ROS-protective effect on Hepg2 cell line [4]. The aqueous extracts showed no cytotoxic activity on HepG2 cells, but they suppressed ROS production induced by H2O2. The antioxidant activity could be due to the chemical composition performed by LC-ESI/LTQOrbitrap/MS [5]. Fourteen compounds were identified as caffeic acid, gallic acid, 3,4-dihydroxybenzoic acid, syringic acid, epigallocatechin, rutin, isoquercetin, quercetin, myricetin, epicatechin, naringin, luteolin, resveratrol and ferulic acid. Our findings suggest the Hura crepitans leaves could be used as nutraceuticals in food preparations.

Chemical characterization and antioxidant activity of Hura crepitans L. leaves

Sinisgalli C.;Labanca F.;Miglionico R.;Vassallo A.;Armentano M. F.;Ostuni A.;Milella L
2019

Abstract

Hura crepitans L. belongs to the family of Euphorbiaceae and it showed many ethnomedicinal applications; the bark extract was used to treat leprosy, eczema and in warfare. Recently a wide range of therapeutic applications of the leaves, stem bark of this plant were reported including astringent, emollient and strong purgative actions, dermatological uses, fungicidal, antimicrobial [1, 2]. The purpose of this study is to increase scientific knowledge on the chemical composition and antioxidant activity of leaves of H. crepitans L. Dried leaves were extracted by different methods: infusion, decoction, maceration by using water as solvent, and sequential extraction by using n-hexane, chloroform, choloform-MeOH 9.1, MeOH was carried out. MeOH extract was partitioned by water and butanol. Antioxidant activity of the extracts was measured by different in-vitro cellfree assays along with the total polyphenolic content and Relative Antioxidant Capacity Index (RACI) was calculated [3]. Aqueous extracts reported the highest RACI values and then, they were tested for the evaluation of cell viability and ROS-protective effect on Hepg2 cell line [4]. The aqueous extracts showed no cytotoxic activity on HepG2 cells, but they suppressed ROS production induced by H2O2. The antioxidant activity could be due to the chemical composition performed by LC-ESI/LTQOrbitrap/MS [5]. Fourteen compounds were identified as caffeic acid, gallic acid, 3,4-dihydroxybenzoic acid, syringic acid, epigallocatechin, rutin, isoquercetin, quercetin, myricetin, epicatechin, naringin, luteolin, resveratrol and ferulic acid. Our findings suggest the Hura crepitans leaves could be used as nutraceuticals in food preparations.
File in questo prodotto:
File Dimensione Formato  
Poster 2019_SINISGALLI_ISSNP Matera 1-5 luglio 2019.pdf

accesso aperto

Descrizione: Program and Abstract
Tipologia: Altro materiale allegato
Licenza: DRM non definito
Dimensione 1.24 MB
Formato Adobe PDF
1.24 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11563/144554
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact