ROLE OF VOCS IN THE INTERACTION OF RHIZOBACTERIA AND PLANT PATHOGENIC FUNGI N. S. Iacobellis, A Giorgio, D. Lamorte, and P. Lo Cantore. Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano, 85100, Potenza, Italy; E-mail: nicola.iacobellis@unibas.it. Among 162 bacteria isolates obtained from bean plants rhixosphere 60 inhibited in dual plate assays the growth of bean fungal and bacterial pathogens. When the above 60 antagonistic bacteria were applied to bean seeds and the relative plants challenged with a highly virulent strain of X. campestris pv. phaseoli var. fuscans six isolates highly protected bacterized bean plants. The bean plant protection by the six bacterial strains, though at different level, was observed either in in vitro or in greenhouse cotyledon and trifoliate pathogenicity leaf assays, respectively. The Induced Systemic Resistance (ISR) is apparently involved and, in fact, ISR has been recently ascertained in parallel studies using the pathosystem Arapidopsis thaliana and X. c. pv. amoriaceae for 3 of the above rhizobacteria. The six antagonistic bacterial strains did produce an array of hydrolytic enzymes, diffusible antimicrobial substances and Volatile Organic Compounds (VOCs) which inhibit the growth of several plant pathogenic fungi as assessed either in dual plate and VOCs assays. In particular, strains of Sclerotinia sclerotiorum resulted highly sensible to both diffusible and volatiles and for that it was selected for further studies. Pure synthetic VOCs such as 2-nonanone, 2-undecanone, m-cymene, limonene, dimethy disulfite, dimethyl trisulfide highly inhibited, though with different MIC, the growth of S. slerotiorum strains and showed haemolytic activity suggesting that membrane systems are the target of the above VOCs. Preliminary observation with optical and electron microscopes of S. sclerotiorum mycelia appear to confirm that membrane target for the above VOCs.

ROLE OF VOCS IN THE INTERACTION OF RHIZOBACTERIA AND PLANT PATHOGENIC FUNGI

IACOBELLIS, Nicola Sante;GIORGIO, ANNALISA;LAMORTE, DANIELA;LO CANTORE, Pietro
2013

Abstract

ROLE OF VOCS IN THE INTERACTION OF RHIZOBACTERIA AND PLANT PATHOGENIC FUNGI N. S. Iacobellis, A Giorgio, D. Lamorte, and P. Lo Cantore. Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano, 85100, Potenza, Italy; E-mail: nicola.iacobellis@unibas.it. Among 162 bacteria isolates obtained from bean plants rhixosphere 60 inhibited in dual plate assays the growth of bean fungal and bacterial pathogens. When the above 60 antagonistic bacteria were applied to bean seeds and the relative plants challenged with a highly virulent strain of X. campestris pv. phaseoli var. fuscans six isolates highly protected bacterized bean plants. The bean plant protection by the six bacterial strains, though at different level, was observed either in in vitro or in greenhouse cotyledon and trifoliate pathogenicity leaf assays, respectively. The Induced Systemic Resistance (ISR) is apparently involved and, in fact, ISR has been recently ascertained in parallel studies using the pathosystem Arapidopsis thaliana and X. c. pv. amoriaceae for 3 of the above rhizobacteria. The six antagonistic bacterial strains did produce an array of hydrolytic enzymes, diffusible antimicrobial substances and Volatile Organic Compounds (VOCs) which inhibit the growth of several plant pathogenic fungi as assessed either in dual plate and VOCs assays. In particular, strains of Sclerotinia sclerotiorum resulted highly sensible to both diffusible and volatiles and for that it was selected for further studies. Pure synthetic VOCs such as 2-nonanone, 2-undecanone, m-cymene, limonene, dimethy disulfite, dimethyl trisulfide highly inhibited, though with different MIC, the growth of S. slerotiorum strains and showed haemolytic activity suggesting that membrane systems are the target of the above VOCs. Preliminary observation with optical and electron microscopes of S. sclerotiorum mycelia appear to confirm that membrane target for the above VOCs.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11563/63802
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