Bacterial species in the genus of Burkholderia have numerous interesting properties of potential industrial applications, including production of antibiotics, biosurfactant, bioplastics, and degradation of environmental contaminants. Bacterial biosynthesis of antibiotics is highly dependent on environmental factors, including types of nutrient, pH, and temperature. Burkholderia gladioli pv. agaricicola (Bga) , plant, fungi and bacteria pathogens, have the ability to produce in vitro secondary metabolites with relevant biological activities. As the nutrient media play un important role on the bacterial growth and on the production of metabolites, in this work the effects of addition of different carbon and nitrogen sources to liquid minimal mineral media (MMA), were investigated in relation to the production of bioactive metabolites. Results showed that the transition into stationary phase and the growth rate of bacteria increased, due to less competition for nutrient components, resulting in high antifungal activity vs. Rhizoctonia solani (Rs) and Bacillus megaterium (Bm). The maximum growth and antimicrobial activity of Bga were obtained after incubation for 13 days at 25 C° by using ammonium nitrate and lactose while the production of metabolites increased of 400%.

INFLUENCE OF NUTRIENT MEDIA ON THE PRODUCTION OF METABOLITES PRODUCED BY BURKHOLDERIA GLADIOLI PV. AGARICICOLA

H. S. Elshafie;IACOBELLIS, Nicola Sante;RACIOPPI, Rocco;LELARIO, FILOMENA;SCRANO, Laura;BUFO, Sabino Aurelio
2012-01-01

Abstract

Bacterial species in the genus of Burkholderia have numerous interesting properties of potential industrial applications, including production of antibiotics, biosurfactant, bioplastics, and degradation of environmental contaminants. Bacterial biosynthesis of antibiotics is highly dependent on environmental factors, including types of nutrient, pH, and temperature. Burkholderia gladioli pv. agaricicola (Bga) , plant, fungi and bacteria pathogens, have the ability to produce in vitro secondary metabolites with relevant biological activities. As the nutrient media play un important role on the bacterial growth and on the production of metabolites, in this work the effects of addition of different carbon and nitrogen sources to liquid minimal mineral media (MMA), were investigated in relation to the production of bioactive metabolites. Results showed that the transition into stationary phase and the growth rate of bacteria increased, due to less competition for nutrient components, resulting in high antifungal activity vs. Rhizoctonia solani (Rs) and Bacillus megaterium (Bm). The maximum growth and antimicrobial activity of Bga were obtained after incubation for 13 days at 25 C° by using ammonium nitrate and lactose while the production of metabolites increased of 400%.
2012
9789892032627
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11563/35043
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