An Investigation into the Possibility of Reusing Wastewater and Sludge on Soil Enriched with Humic Acid of Young Growing Seedlings of Cupressus Arizonica Greene

The influence of Humic acids (HA) in combination to Treated Municipal Waste Water (TMWW) and biological sludge (S) reuse was studied, for the characteristics of growth and metal accumulation in the forestal species Cupressus Arizonica Greene and soil characteristics. The experiment was held at the forestal seedbed of Agrinio at an experimental spacing of random groups containing 12 combinations of TMWW, S and HA plus control, in 4 replicates, meaning 12x4. The findings were: The interventions affected statistically significant the micronutrients capacity of Cu, Fe, Zn, and Mn of soil, leaves and roots. Humic acids raised significantly the availability of Cu and Zn micronutrients in the soil, but did not affect their concentrations in leaves and roots. The higher concentration of Cu and Zn in the leaves was found in the absence of humics with the intervention of E1 (control), while in the presence of humics with the intervention of E11 (Control+TMWW+200mg/kg HA). In the roots the higher concentration of Cu and Zn was achieved in the absence of humics with the intervention of E1 (control), while in the presence of humics with the intervention of E2 (Control+200mg/kg HA). Closing, in the soil, the highest concentration of Cu and Zn was observed in the absence of humics with the intervention of E10 (control+S), while in the presence of humics with the intervention of E8 (TMWW+S+200mg/kg HA).