Bovine oocytes and embryos were exposed to increasing levels of urea (i.e., 2-4-6 and 8 mM) for 24 hrs during in vitro maturation, fertilization and (day-2) embryo culture. This caused a dose-dependant decrease of cleavage and blastocyst rates in all the in phases. A higher urea sensitivity was found during the oocyte in vitro maturation with statistically significant (P< 0.05) decreases of cleavage and blastocyst rates at 8 and 6 mM, respectively.
Effetti dell’urea sullo sviluppo embrionale in vitro nella specie bovina.
BONI, Raffaele;LANGELLA, Michele;
2004-01-01
Abstract
Bovine oocytes and embryos were exposed to increasing levels of urea (i.e., 2-4-6 and 8 mM) for 24 hrs during in vitro maturation, fertilization and (day-2) embryo culture. This caused a dose-dependant decrease of cleavage and blastocyst rates in all the in phases. A higher urea sensitivity was found during the oocyte in vitro maturation with statistically significant (P< 0.05) decreases of cleavage and blastocyst rates at 8 and 6 mM, respectively.File in questo prodotto:
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