Integrated transcriptomic and proteomic approach to identify the major Toxoneuron nigriceps venom proteins Introduction: Toxoneuron nigriceps is an endophagous parasitoid of the tobacco budworm Heliothis virescens. Upon parasitizaztion adult female injects into the host body, along with the egg, the venom and the ovarian calyx fluid contains a symbiotic polydnavirus and ovarian proteins. These maternal secretions along with embryonic factors, the teratocytes, are the main host regulation factors, involved in the disruption of host immune system in order to ensure a appropriate environment for the progeny development (Pennacchio et al. 2001; Pennacchio & Strand 2006). To date the role of T. nigricepsvenom is unknown although preliminary information indicates an involvement in early suppression of host immune response. Methods: To identify the main T. nigriceps venom compounds a combined transcriptomic and proteomic approach was used. From de novo assembly of all cDNA, 17742 contigs were obtained; all sequences were analyzed using Blast2GO software. The sequences analyzed display enrichment in genes that encode for similar known venom components but also provide the first sketch of cellular components, molecular functions, biological processes and some unique sequences of T. nigriceps venom gland. For the proteome analyses, the T. nigricepsvenom was fractionated by two-dimensional gel electrophoresis; several spots were trypsinized and analyzed by High Performance Liquid Chromatography-Tandem Mass Spectrometry. Results/Conclusions: The tandem-mass spectrometric data analysis, obtained using MASCOT software, allowed the identification of a large number of proteins in the T. nigriceps venom. To the best of our knowledge this is the first study targeted to the transcriptomic and proteomic analysis of the T. nigriceps venom.
Integrated transcriptomic and proteomic approach to identify the major Toxoneuron nigriceps venom proteins
GROSSI, GERARDA;LAURINO, SIMONA;SALVIA, ROSANNA;BUFO, Sabino Aurelio;FALABELLA, Patrizia
2016-01-01
Abstract
Integrated transcriptomic and proteomic approach to identify the major Toxoneuron nigriceps venom proteins Introduction: Toxoneuron nigriceps is an endophagous parasitoid of the tobacco budworm Heliothis virescens. Upon parasitizaztion adult female injects into the host body, along with the egg, the venom and the ovarian calyx fluid contains a symbiotic polydnavirus and ovarian proteins. These maternal secretions along with embryonic factors, the teratocytes, are the main host regulation factors, involved in the disruption of host immune system in order to ensure a appropriate environment for the progeny development (Pennacchio et al. 2001; Pennacchio & Strand 2006). To date the role of T. nigricepsvenom is unknown although preliminary information indicates an involvement in early suppression of host immune response. Methods: To identify the main T. nigriceps venom compounds a combined transcriptomic and proteomic approach was used. From de novo assembly of all cDNA, 17742 contigs were obtained; all sequences were analyzed using Blast2GO software. The sequences analyzed display enrichment in genes that encode for similar known venom components but also provide the first sketch of cellular components, molecular functions, biological processes and some unique sequences of T. nigriceps venom gland. For the proteome analyses, the T. nigricepsvenom was fractionated by two-dimensional gel electrophoresis; several spots were trypsinized and analyzed by High Performance Liquid Chromatography-Tandem Mass Spectrometry. Results/Conclusions: The tandem-mass spectrometric data analysis, obtained using MASCOT software, allowed the identification of a large number of proteins in the T. nigriceps venom. To the best of our knowledge this is the first study targeted to the transcriptomic and proteomic analysis of the T. nigriceps venom.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
