Diet high in fatty acids increases plasma total cholesterol and lowdensity lipoprotein (LDL) concentration causing hyperlipidemia and atherosclerotic cardiovascular disease, one of the major causes of death worldwide. Cholesterol homeostasis in the body is maintained mainly by de novo synthesis, intestinal absorption, and biliary and fecal excretion [1]. Natural products could influence the lipid transporter expression and related diseases [2]. In the present work, the biological activity of Capsicum annuum L. cv Senise (Solanaceae), a sweet dried pepper of Basilicata region (Italy), was investigated in HepG2 and Caco2 cells used as liver and intestine model, respectively. The extract showed no cytotoxic effect on both cell line after 24 and 48h evaluated by MTT assay. Then, cells were treated with oleic acid (OA) in presence or absence of different concentrations of C. annuun extract (CAE) and the expression of lipid transporters was evaluated by RTqPCR and western blotting. CAE promoted ABCA1 expression in OAtreated HepG2 cell line, a transporter involved in cholesterol reuptake from peripheric tissues. CAE also affected lipid transport through intestinal barrier. Particularly, it drastically upregulated ABCA1, localized on basolateral membrane of enterocytes, facilitating the efflux of cholesterol to circulation as constituent of HDL, together with ABCG5 and ABCG8, two ABC transporters which promote efflux of cellular cholesterol to intestinal lumen and hepatobiliary secretion. In addition, CAE promoted the expression of LDL receptor in Caco2 cells. These results suggest as C. annuum cv Senise could be helpful in lowering plasma cholesterol levels and could play an important role in hyperlipidemia ranked as one of the greatest risk factors contributing to prevalence and severity of coronary heart diseases. Previously published in: [1] Yu XH et al. (2014) Clin. Chim. Acta, 428, 8288 [2] ElTantawy, WH et al. (2019) Arch Physiol Biochem, 125(2), 128135
Impact of Capsicum annuum L. cv Senise on cholesterol transporters
Sinisgalli C.;Milella L.;Russo D.;Ostuni A.
2021-01-01
Abstract
Diet high in fatty acids increases plasma total cholesterol and lowdensity lipoprotein (LDL) concentration causing hyperlipidemia and atherosclerotic cardiovascular disease, one of the major causes of death worldwide. Cholesterol homeostasis in the body is maintained mainly by de novo synthesis, intestinal absorption, and biliary and fecal excretion [1]. Natural products could influence the lipid transporter expression and related diseases [2]. In the present work, the biological activity of Capsicum annuum L. cv Senise (Solanaceae), a sweet dried pepper of Basilicata region (Italy), was investigated in HepG2 and Caco2 cells used as liver and intestine model, respectively. The extract showed no cytotoxic effect on both cell line after 24 and 48h evaluated by MTT assay. Then, cells were treated with oleic acid (OA) in presence or absence of different concentrations of C. annuun extract (CAE) and the expression of lipid transporters was evaluated by RTqPCR and western blotting. CAE promoted ABCA1 expression in OAtreated HepG2 cell line, a transporter involved in cholesterol reuptake from peripheric tissues. CAE also affected lipid transport through intestinal barrier. Particularly, it drastically upregulated ABCA1, localized on basolateral membrane of enterocytes, facilitating the efflux of cholesterol to circulation as constituent of HDL, together with ABCG5 and ABCG8, two ABC transporters which promote efflux of cellular cholesterol to intestinal lumen and hepatobiliary secretion. In addition, CAE promoted the expression of LDL receptor in Caco2 cells. These results suggest as C. annuum cv Senise could be helpful in lowering plasma cholesterol levels and could play an important role in hyperlipidemia ranked as one of the greatest risk factors contributing to prevalence and severity of coronary heart diseases. Previously published in: [1] Yu XH et al. (2014) Clin. Chim. Acta, 428, 8288 [2] ElTantawy, WH et al. (2019) Arch Physiol Biochem, 125(2), 128135I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
