The aim of this work was to study the electrophoretic behaviour of Oenococcus oeni (O. oeni) under the effect of ethanol stress. The bacterial outer surface of O. oeni is rich of ionizable groups so the changes in the charging rates in the optimal and stressed conditions could be evaluated by capillary electrophoresis. As a first attempt, it was necessary to optimize the electrophoretic conditions for the identification and efficient separation of this microorganism by capillary electrophoresis. After this preliminary study, the electropherograms show significant differences between cells stressed by ethanol and cells growth in optimal condition. Interestingly, it was observed a substantial difference in electrophoretic profile among different O. oeni strains. The experimental results confirmed the power of capillary electrophoresis for microbial analysis (characterization and separation of microorganisms) since permitting rapid, easy and highly sensitive microbial analysis at low costs for several biological samples.

CAPILLARY ELECTROPHORESIS ANALYSIS OF STRESSED Oenococcus oeni

CIRIELLO, Rosanna;GUERRIERI, Antonio;CAFARO, CATERINA;BONOMO, MARIA GRAZIA;SALZANO, Giovanni
2012-01-01

Abstract

The aim of this work was to study the electrophoretic behaviour of Oenococcus oeni (O. oeni) under the effect of ethanol stress. The bacterial outer surface of O. oeni is rich of ionizable groups so the changes in the charging rates in the optimal and stressed conditions could be evaluated by capillary electrophoresis. As a first attempt, it was necessary to optimize the electrophoretic conditions for the identification and efficient separation of this microorganism by capillary electrophoresis. After this preliminary study, the electropherograms show significant differences between cells stressed by ethanol and cells growth in optimal condition. Interestingly, it was observed a substantial difference in electrophoretic profile among different O. oeni strains. The experimental results confirmed the power of capillary electrophoresis for microbial analysis (characterization and separation of microorganisms) since permitting rapid, easy and highly sensitive microbial analysis at low costs for several biological samples.
2012
9788890767081
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11563/36633
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