A simple, rapid and sensitive HPLC method for trace determination of resorcinol in body fluids is described. It uses a reversed phase C18 column and isocratic elution with amperometric detection on glassy carbon at +1.0 V vs. Ag/AgCl reference electrode. 3,5-dihydroxytoluol was used as internal standard. Sample treatment consists of a simple microcolumn extraction step requiring sample volumes as low as 200 microliters. The proposed method gives detection limits (4 ng/mL and 5 ng/mL in serum and urine respectively) about two orders of magnitude lower than other HPLC methods already reported and should prove useful for studying dermal adsorption and metabolic disposition of topically applied resorcinol.
Determination of free resorcinol in human serum and urine by high performance liquid chromatography with amperometric detection
GUERRIERI, Antonio;
1986-01-01
Abstract
A simple, rapid and sensitive HPLC method for trace determination of resorcinol in body fluids is described. It uses a reversed phase C18 column and isocratic elution with amperometric detection on glassy carbon at +1.0 V vs. Ag/AgCl reference electrode. 3,5-dihydroxytoluol was used as internal standard. Sample treatment consists of a simple microcolumn extraction step requiring sample volumes as low as 200 microliters. The proposed method gives detection limits (4 ng/mL and 5 ng/mL in serum and urine respectively) about two orders of magnitude lower than other HPLC methods already reported and should prove useful for studying dermal adsorption and metabolic disposition of topically applied resorcinol.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
