Chemically modified electrodes obtained by direct interaction between gold substrate and tetracyanonickelate(II) ions in alkaline medium were characterized by cyclic voltammetry toward the electrooxidation of carbohydrates. Satisfactory performance with respect to catalytic stability, background current stability and lowering of detection limit was obtained for the analysis of ten oligosaccharides contained in a synthetic sample by anion-exchange chromatography in alkaline solutions. Under constant applied potential of 0.55V (vs. Ag/AgCl), the detection limits (S/N=3) for all investigated compounds ranged between 3 and 6 pmol injected for melezitose and turanose, respectively. The dynamic linear ranges spanned generally three orders of magnitude above the limit of detection. The effects of the concentration of tetracyanonickelate(II) ion on the catalytic activity, amperometric stability and chromatographic separations were examined.
ELECTROCATALYSIS AND DETECTION OF CARBOHYDRATES BY ANION-EXCHANGE CHROMATOGRAPHY AT A GOLD SUBSTRATE ELECTRODE MODIFIED WITH NICKEL CYANIDE IONS.
CASELLA, Innocenzo Giuseppe;
2001-01-01
Abstract
Chemically modified electrodes obtained by direct interaction between gold substrate and tetracyanonickelate(II) ions in alkaline medium were characterized by cyclic voltammetry toward the electrooxidation of carbohydrates. Satisfactory performance with respect to catalytic stability, background current stability and lowering of detection limit was obtained for the analysis of ten oligosaccharides contained in a synthetic sample by anion-exchange chromatography in alkaline solutions. Under constant applied potential of 0.55V (vs. Ag/AgCl), the detection limits (S/N=3) for all investigated compounds ranged between 3 and 6 pmol injected for melezitose and turanose, respectively. The dynamic linear ranges spanned generally three orders of magnitude above the limit of detection. The effects of the concentration of tetracyanonickelate(II) ion on the catalytic activity, amperometric stability and chromatographic separations were examined.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
